Evaluation of a real-time PCR assay for diagnosis of schistosomiasis japonica in the domestic goat.

ABSTRACT:

Background

Schistosomiasis japonica is an infectious disease caused by Schistosoma japonicum that seriously endangers human health. Domestic animals have important roles in disease transmission and goats are considered a primary reservoir host and source of infection. The prevalence and intensity of schistosomiasis infections have significantly decreased in China, and a more sensitive, specific detection method is urgently needed. The aim of this study was to develop a real-time PCR assay for accurate detection of S. japonicum infection in goats.

Methods

A real-time PCR method for detecting schistosomiasis japonica in goats was developed by amplification of a specific S. japonicum DNA fragment, and validated using a total of 94 negative and 159 positive plasma and serum samp

SUBMITTER: Guo Q 

PROVIDER: S-EPMC7590668 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature