Genetic and Functional Characterization of HIV-1 Vpu from HIV-1-Infected North Indian Population.

ABSTRACT: Acquired immunodeficiency syndrome is a pandemic disease due to increased variability in causative agent in global distribution; it is attributed to various complications in developing the vaccine, namely, error-prone reverse transcriptase, rapid replication, and high recombination rate. Vpu downmodulates CD4 in infected cells, and it targets the newly synthesized CD4 molecules from the endoplasmic reticulum. The aim of this study was to identify the level of genetic changes in the Vpu gene from HIV-1-infected North Indian individuals and determine the functional relevance with respect to the CD4 downregulation potential of this protein. Genomic DNA was isolated from peripheral blood mononuclear cells, and the Vpu gene was polymerase chain reaction amplified with specific pri

SUBMITTER: Singh J 

PROVIDER: S-EPMC7590825 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature