Critical role of WNK1 in MYC-dependent early mouse thymocyte development.

ABSTRACT: WNK1, a kinase that controls kidney salt homeostasis, also regulates adhesion and migration in CD4⁺ T cells. Wnk1 is highly expressed in thymocytes, and since migration is important for thymocyte maturation, we investigated a role for WNK1 in mouse thymocyte development. We find that WNK1 is required for the transition of double negative (DN) thymocytes through the β-selection checkpoint and subsequent proliferation and differentiation into double positive (DP) thymocytes. Furthermore, we show that WNK1 negatively regulates LFA1-mediated adhesion and positively regulates CXCL12-induced migration in DN thymocytes. Despite this, migration defects of WNK1-deficient thymocytes do not account for the developmental arrest. Instead, we show that in DN thymocytes WNK1 transduces

SUBMITTER: Kochl R 

PROVIDER: S-EPMC7591260 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature