Chemically Defined, High-Density Insect Cell-Based Expression System for Scalable AAV Vector Production.

ABSTRACT: The recombinant adeno-associated virus (AAV) vector is one of the most utilized viral vectors in gene therapy due to its robust, long-term in vivo transgene expression and low toxicity. One major hurdle for clinical AAV applications is large-scale manufacturing. In this regard, the baculovirus-based AAV production system is highly attractive due to its scalability and predictable biosafety. Here, we describe a simple method to improve the baculovirus-based AAV production using the ExpiSf Baculovirus Expression System with a chemically defined medium for suspension culture of high-density ExpiSf9 cells. Baculovirus-infected ExpiSf9 cells produced up to 5 × 10¹¹ genome copies of highly purified AAV vectors per 1 mL of suspension culture, which is up to a 19-fold higher yiel

SUBMITTER: Kurasawa JH 

PROVIDER: S-EPMC7591331 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature