Epithelial layer unjamming shifts energy metabolism toward glycolysis.

ABSTRACT: In development of an embryo, healing of a wound, or progression of a carcinoma, a requisite event is collective epithelial cellular migration. For example, cells at the advancing front of a wound edge tend to migrate collectively, elongate substantially, and exert tractions more forcefully compared with cells many ranks behind. With regards to energy metabolism, striking spatial gradients have recently been reported in the wounded epithelium, as well as in the tumor, but within the wounded cell layer little is known about the link between mechanical events and underlying energy metabolism. Using the advancing confluent monolayer of MDCKII cells as a model system, here we report at single cell resolution the evolving spatiotemporal fields of cell migration speeds, cell shapes, and traction

SUBMITTER: DeCamp SJ 

PROVIDER: S-EPMC7591531 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature