Interplay among p21^Waf1/Cip1, MUSASHI-1 and Kruppel-like factor 4 in activation of Bmi1-Cre^ER reserve intestinal stem cells after gamma radiation-induced injury.

ABSTRACT: Gamma radiation is a commonly used adjuvant treatment for abdominally localized cancer. Since its therapeutic potential is limited due to gastrointestinal (GI) syndrome, elucidation of the regenerative response following radiation-induced gut injury is needed to develop a preventive treatment. Previously, we showed that Krüppel-like factor 4 (KLF4) activates certain quiescent intestinal stem cells (ISCs) marked by Bmi1-Cre^ER to give rise to regenerating crypts following γ irradiation. In the current study, we showed that γ radiation-induced expression of p21^Waf1/Cip1 in Bmi1-Cre^ER cells is likely mitigated by MUSASHI-1 (MSI1) acting as a negative regulator of p21^Waf1/Cip1 mRNA translation, which promotes exit of the Bmi1-Cre^ER cells fr

SUBMITTER: Orzechowska EJ 

PROVIDER: S-EPMC7591575 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature