E2F7-EZH2 axis regulates PTEN/AKT/mTOR signalling and glioblastoma progression.

ABSTRACT:

Background

E2F transcription factors are considered to be important drivers of tumour growth. E2F7 is an atypical E2F factor, and its role in glioblastoma remains undefined.

Methods

E2F7 expression was examined in patients by IHC and qRT-PCR. The overall survival probability was determined by statistical analyses. MTT assay, colony formation, cell-cycle assay, cell metastasis and the in vivo model were employed to determine the functional role of E2F7 in glioblastoma. Chromatin immunoprecipitation, luciferase assay and western blot were used to explore the underlying mechanisms.

Results

E2F7 was found to be up-regulated in glioblastoma patients, and high E2F7 expression was associated with poor overall survival in glioblastoma patients. Functional studies showed that

SUBMITTER: Yang R 

PROVIDER: S-EPMC7591888 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature