Force-clamp spectroscopy identifies a catch bond mechanism in a Gram-positive pathogen.

ABSTRACT: Physical forces have profound effects on cellular behavior, physiology, and disease. Perhaps the most intruiguing and fascinating example is the formation of catch-bonds that strengthen cellular adhesion under shear stresses. Today mannose-binding by the Escherichia coli FimH adhesin remains one of the rare microbial catch-bond thoroughly characterized at the molecular level. Here we provide a quantitative demonstration of a catch-bond in living Gram-positive pathogens using force-clamp spectroscopy. We show that the dock, lock, and latch interaction between staphylococcal surface protein SpsD and fibrinogen is strong, and exhibits an unusual catch-slip transition. The bond lifetime first grows with force, but ultimately decreases to behave as a slip bond beyond a critical force (~1 nN) th

SUBMITTER: Mathelie-Guinlet M 

PROVIDER: S-EPMC7591895 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature