MicroRNA-499 rs3746444 polymorphism in Egyptian children with biliary atresia.

ABSTRACT:

Aim of the study

We aimed to evaluate the association of microRNA-499 rs3746444 polymorphism and biliary atresia (BA) risk and its correlation with clinic-pathologic features of BA.

Material and methods

This study was performed on 300 Egyptian children (100 BA cases, 100 cases with cholestatic liver diseases other than BA and 100 healthy controls). Routine laboratory investigations, clinical examination and abdominal ultrasound were done. All infants were genotyped for miR-499 single nucleotide polymorphisms (SNPs) (rs3746444 A>G) by real-time polymerase chain reaction (PCR) fluorescence detection on a Rotor Gene Real Time PCR System (QIAGEN, GmbH) using fluorescent labeled probes.

Results

The AG genotype was the most prevalent genotype of miR-499 rs3746444 among the

SUBMITTER: Gawish E 

PROVIDER: S-EPMC7592094 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature