Monoclonal antibody specific to the Di^a blood group antigen generated by phage display technology.

ABSTRACT:

Background

Alloanti-Di^a can be implicated in mild to severe blood transfusion reactions. Given the concomitance of a high prevalence of the Di^a antigen and antibody circulating in some populations, an anti-Di^a typing reagent is required in order to enable safe blood transfusions. Limitations of hybridoma technology to produce such a reagent led to the use of phage display technology to generate an anti-Di^a monoclonal antibody.

Materials and methods

A library of phages displaying murine single-chain variable fragment antibody (scFv-phages) was consecutively adsorbed with different panels of Di(a-b+) red cells to eliminate scFc-phages that potentially bind irrelevant blood group antigens. Thereafter, the subtractive library was speci

SUBMITTER: Thattanon P 

PROVIDER: S-EPMC7592165 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature