Regulatory coupling between long noncoding RNAs and senescence in irradiated microglia.

ABSTRACT:

Background

Microglia have been implicated in the pathogenesis of radiation-induced brain injury (RIBI), which severely influences the quality of life during long-term survival. Recently, irradiated microglia were speculated to present an aging-like phenotype. Long noncoding RNAs (lncRNAs) have been recognized to regulate a wide spectrum of biological processes, including senescence; however, their potential role in irradiated microglia remains largely uncharacterized.

Methods

We used bioinformatics and experimental methods to identify and analyze the senescence phenotype of irradiated microglia. Western blotting, enzyme-linked immunosorbent assays, immunofluorescence, and quantitative real-time reverse transcription-polymerase chain reaction were performed to clarify the rel

SUBMITTER: Xu A 

PROVIDER: S-EPMC7592596 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature