Development and validation of an assay for detection of Japanese encephalitis virus specific antibody responses.

ABSTRACT:

Introduction

Although immune responses to the Japanese Encephalitis virus (JEV), and the dengue viruses (DENV) have a potential to modulate the immune responses to each other, this has been poorly investigated. Therefore, we developed an ELISA to identify JEV specific, DENV non cross-reactive antibody responses by identifying JEV specific, highly conserved regions of the virus and proceeded to investigate if the presence of JEV specific antibodies associate with dengue disease severity.

Methodology and results

22 JEV specific peptides were identified from highly conserved regions of the virus and the immunogenicity and specificity of these peptides were assessed in individuals who were non-immune to JEV and DENV (JEV-DENV-, N = 30), those who were only immune to the JEV and

SUBMITTER: Pushpakumara PD 

PROVIDER: S-EPMC7592747 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature