An electrochemical biosensor for the detection of Mycobacterium tuberculosis DNA from sputum and urine samples.

ABSTRACT: Tuberculosis (TB) is a major global public health problem with high mortality and morbidity. In low-middle income countries (LMIC) a large number of respiratory symptomatic cases that require TB screening per year demands more accurate, fast and affordable testing for TB diagnostics. Sputum smear is the initial screening test in LMICs, however, its sensitivity is limited in patients with low sputum bacilli load. The same limitation is observed in the currently available molecular tests. We designed, standardized and evaluated an electrochemical biosensor that detects the highly specific DNA insertion element 6110 (IS6110). A PCR amplified DNA product is hybridized on the surface of the working electrode built on FTO-Glass with immobilized specific DNA probes, after which cyclic voltammetry

SUBMITTER: Ramos-Sono D 

PROVIDER: S-EPMC7592764 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature