Landscape of N⁶-Methyladenosine Modification Patterns in Human Ameloblastoma.

ABSTRACT:

Objective

To comprehensively analyze the global N⁶-methyladenosine (m⁶A) modification pattern in ameloblastoma.

Methods

m⁶A peaks in ameloblastoma and normal oral tissues were detected by MeRIP-seq. Differentially methylated m⁶A sites within messenger RNAs (mRNAs), long no-coding RNA (lncRNAs) and circular RNA (circRNAs) were identified, followed by functional enrichment analysis. By comprehensively analyzing MeRIP-seq and RNA-seq data, differentially expressed mRNAs, lncRNAs and circRNAs containing differentially methylated sites were identified. RNA binding proteins (RBPs) were then identified for differentially methylated m⁶A sites.

Results

In total, 3,673 differentially methylated m⁶A sites within c

SUBMITTER: Niu X 

PROVIDER: S-EPMC7592903 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature