A Partial C₄ Photosynthetic Biochemical Pathway in Rice.

ABSTRACT: Introduction of a C₄ photosynthetic pathway into C₃ rice (Oryza sativa) requires installation of a biochemical pump that concentrates CO₂ at the site of carboxylation in modified bundle sheath cells. To investigate the feasibility of this, we generated a quadruple line that simultaneously accumulates four of the core C₄ photosynthetic enzymes from the NADP-malic enzyme subtype, phosphoenolpyruvate carboxylase (ZmPEPC), NADP-malate dehydrogenase (ZmNADP-MDH), NADP-malic enzyme (ZmNADP-ME), and pyruvate phosphate dikinase (ZmPPDK). This led to enhanced enzyme activity and mild phenotypic perturbations but was largely neutral in its effects on photosynthetic rate. Measurements of the flux of ¹³CO<

SUBMITTER: Lin H 

PROVIDER: S-EPMC7593541 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature