Full Transcriptome Analysis of Callus Suspension Culture System of Bletilla striata.

ABSTRACT:

Background

Bletilla striata has been widely used in the pharmacology industry. To effectively produce the secondary metabolites through suspension cultured cells of B. striata, it is important to exploring the full-length transcriptome data and the genes related to cell growth and chemical producing of all culture stages. We applied a combination of Real-Time Sequencing of Single Molecule (SMRT) and second-generation sequencing (SGS) to generate the complete and full-length transcriptome of B. striata suspension cultured cells.

Methods

The B. striata transcriptome was formed in de novo way by using PacBio isoform sequencing (Iso-Seq) on a pooled RNA sample derived from 23 samples of 10 culture stages, to explore the potential for capturing

SUBMITTER: Li L 

PROVIDER: S-EPMC7593603 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature