A unified design allows fine-tuning of biosensor parameters and application across bacterial species.

ABSTRACT: In recent years, transcriptional biosensors have become valuable tools in metabolic engineering as they allow semiquantitative determination of metabolites in single cells. Although being perfectly suitable tools for high-throughput screenings, application of transcriptional biosensors is often limited by the intrinsic characteristics of the individual sensor components and their interplay. In addition, biosensors often fail to work properly in heterologous host systems due to signal saturation at low intracellular metabolite concentrations, which typically limits their use in high-level producer strains at advanced engineering stages. We here introduce a biosensor design, which allows fine-tuning of important sensor parameters and restores the sensor response in a heterologous expression

SUBMITTER: Sonntag CK 

PROVIDER: S-EPMC7593625 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature