Heterologous expression of plant glycosyltransferases for biochemistry and structural biology.

ABSTRACT: Much of the carbon captured by photosynthesis is converted into the polysaccharides that constitute plant cell walls. These complex macrostructures are composed of cellulose, hemicellulose, and pectins, together with small amounts of structural proteins, minerals, and in many cases lignin. Wall components assemble and interact with one another to produce dynamic structures with many capabilities, including providing mechanical support to plant structures and determining plant cell shape and size. Despite their abundance, major gaps in our knowledge of the synthesis of the building blocks of these polymers remain, largely due to ineffective methods for expression and purification of active synthetic enzymes for in vitro biochemical analyses. The hemicellulosic polysaccharide, xyloglucan, co

SUBMITTER: Prabhakar PK 

PROVIDER: S-EPMC7593805 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature