Phage-Assisted Continuous Evolution (PACE): A Guide Focused on Evolving Protein-DNA Interactions.

ABSTRACT: The uptake of directed evolution methods is increasing, as these powerful systems can be utilized to develop new biomolecules with altered/novel activities, for example, proteins with new catalytic functions or substrate specificities and nucleic acids that recognize an intended target. Especially useful are systems that incorporate continuous evolution, where the protein under selective pressure undergoes continuous mutagenesis with little-to-no input from the researcher once the system is started. However, continuous evolution methods can be challenging to implement and a daunting investment of time and resources. Our intent is to provide basic information and helpful suggestions that we have gained from our experience with bacterial phage-assisted continuous evolution (PACE) towa

SUBMITTER: Popa SC 

PROVIDER: S-EPMC7593997 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature