An Alcohol Dehydrogenase 3 (ADH3) from Entamoeba histolytica Is Involved in the Detoxification of Toxic Aldehydes.

ABSTRACT: Recently, a putative alcohol dehydrogenase 3, termed EhADH3B of the Entamoeba histolytica isolate HM-1:IMSS was identified, which is expressed at higher levels in non-pathogenic than in pathogenic amoebae and whose overexpression reduces the virulence of pathogenic amoebae. In an in silico analysis performed in this study, we assigned EhADH3B to a four-member ADH3 family, with ehadh3b present as a duplicate (ehadh3b^a/ehadh3b^b). In long-term laboratory cultures a mutation was identified at position 496 of ehadh3b^a, which codes for a stop codon, which was not the case for amoebae isolated from human stool samples. When using transfectants that overexpress or silence ehadh3b^b, we found no or little ef

SUBMITTER: Konig C 

PROVIDER: S-EPMC7594077 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature