Deep mutagenesis in the study of COVID-19: a technical overview for the proteomics community.

ABSTRACT:

Introduction

The spike (S) of SARS coronavirus 2 (SARS-CoV-2) engages angiotensin-converting enzyme 2 (ACE2) on a host cell to trigger viral-cell membrane fusion and infection. The extracellular region of ACE2 can be administered as a soluble decoy to compete for binding sites on the receptor-binding domain (RBD) of S, but it has only moderate affinity and efficacy. The RBD, which is targeted by neutralizing antibodies, may also change and adapt through mutation as SARS-CoV-2 becomes endemic, posing challenges for therapeutic and vaccine development.

Areas covered

Deep mutagenesis is a Big Data approach to characterizing sequence variants. A deep mutational scan of ACE2 expressed on human cells identified mutations that increase S affinity and guided the engineering of a pot

SUBMITTER: Procko E 

PROVIDER: S-EPMC7594187 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature