Next-generation sequencing of 35 RHD variants in 16 253 serologically D- pregnant women in the Finnish population.

ABSTRACT: Fetal RHD screening for targeted routine antenatal anti-D prophylaxis has been implemented in many countries, including Finland, since the 2010s. Comprehensive knowledge of the RHD polymorphism in the population is essential for the performance and safety of the anti-D prophylaxis program. During the first 3 years of the national screening program in Finland, over 16 000 samples from RhD- women were screened for fetal RHD; among them, 79 samples (0.5%) containing a maternal variant allele were detected. Of the detected maternal variants, 35 cases remained inconclusive using the traditional genotyping methods and required further analysis by next-generation sequencing (NGS) of the whole RHD gene to uncover the variant allele. In addition to the 13 RHD variants that have been previously repo

SUBMITTER: Tammi SM 

PROVIDER: S-EPMC7594403 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature