Modeling glioblastoma invasion using human brain organoids and single-cell transcriptomics.

ABSTRACT:

Background

Glioblastoma (GBM) consists of devastating neoplasms with high invasive capacity, which have been difficult to study in vitro in a human-derived model system. Therapeutic progress is also limited by cellular heterogeneity within and between tumors, among other factors such as therapy resistance. To address these challenges, we present an experimental model using human cerebral organoids as a scaffold for patient-derived GBM cell invasion.

Methods

This study combined tissue clearing and confocal microscopy with single-cell RNA sequencing of GBM cells before and after co-culture with organoid cells.

Results

We show that tumor cells within organoids extend a network of long microtubes, recapitulating the in vivo behavior of GBM. Transcriptional changes implica

SUBMITTER: Krieger TG 

PROVIDER: S-EPMC7594554 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature