Dataset Information

Susceptibility of swine cells and domestic pigs to SARS-CoV-2.

ABSTRACT: The emergence of SARS-CoV-2 has resulted in an ongoing global pandemic with significant morbidity, mortality, and economic consequences. The susceptibility of different animal species to SARS-CoV-2 is of concern due to the potential for interspecies transmission, and the requirement for pre-clinical animal models to develop effective countermeasures. In the current study, we determined the ability of SARS-CoV-2 to (i) replicate in porcine cell lines, (ii) establish infection in domestic pigs via experimental oral/intranasal/intratracheal inoculation, and (iii) transmit to co-housed naïve sentinel pigs. SARS-CoV-2 was able to replicate in two different porcine cell lines with cytopathic effects. Interestingly, none of the SARS-CoV-2-inoculated pigs showed evidence of clinical signs, viral replication or SARS-CoV-2-specific antibody responses. Moreover, none of the sentinel pigs displayed markers of SARS-CoV-2 infection. These data indicate that although different porcine cell lines are permissive to SARS-CoV-2, five-week old pigs are not susceptible to infection via oral/intranasal/intratracheal challenge. Pigs are therefore unlikely to be significant carriers of SARS-CoV-2 and are not a suitable pre-clinical animal model to study SARS-CoV-2 pathogenesis or efficacy of respective vaccines or therapeutics.

SUBMITTER: Meekins DA

PROVIDER: S-EPMC7594707 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Susceptibility of swine cells and domestic pigs to SARS-CoV-2.

Meekins David A DA Morozov Igor I Trujillo Jessie D JD Gaudreault Natasha N NN Bold Dashzeveg D Carossino Mariano M Artiaga Bianca L BL Indran Sabarish V SV Kwon Taeyong T Balaraman Velmurugan V Madden Daniel W DW Feldmann Heinz H Henningson Jamie J Ma Wenjun W Balasuriya Udeni B R UBR Richt Juergen A JA

Emerging microbes & infections 20201201 1

The emergence of SARS-CoV-2 has resulted in an ongoing global pandemic with significant morbidity, mortality, and economic consequences. The susceptibility of different animal species to SARS-CoV-2 is of concern due to the potential for interspecies transmission, and the requirement for pre-clinical animal models to develop effective countermeasures. In the current study, we determined the ability of SARS-CoV-2 to (i) replicate in porcine cell lines, (ii) establish infection in domestic pigs via ...[more]

PMID: 33003988

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Dataset Information

Susceptibility of swine cells and domestic pigs to SARS-CoV-2.

Publications

Susceptibility of swine cells and domestic pigs to SARS-CoV-2.

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