A method for differentiating human induced pluripotent stem cells toward functional cardiomyocytes in 96-well microplates.

ABSTRACT: The capacity of pluripotent stem cells both for self-renewal and to differentiate into any cell type have made them a powerful tool for studying human disease. Protocols for efficient differentiation towards cardiomyocytes using defined, serum-free culture medium combined with small molecules have been developed, but thus far, limited to larger formats. We adapted protocols for differentiating human pluripotent stem cells to functional human cardiomyocytes in a 96-well microplate format. The resulting cardiomyocytes expressed cardiac specific markers at the transcriptional and protein levels and had the electrophysiological properties that confirmed the presence of functional cardiomyocytes. We suggest that this protocol provides an incremental improvement and one that reduces the impact o

SUBMITTER: Balafkan N 

PROVIDER: S-EPMC7595118 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature