Human milk microbiota in sub-acute lactational mastitis induces inflammation and undergoes changes in composition, diversity and load.

ABSTRACT: Sub-acute mastitis (SAM) is a prevalent disease among lactating women, being one of the main reasons for early weaning. Although the etiology and diagnosis of acute mastitis (AM) is well established, little is known about the underlying mechanisms causing SAM. We collected human milk samples from healthy and SAM-suffering mothers, during the course of mastitis and after symptoms disappeared. Total (DNA-based) and active (RNA-based) microbiota were analysed by 16S rRNA gene sequencing and qPCR. Furthermore, mammary epithelial cell lines were exposed to milk pellets, and levels of the pro-inflammatory interleukin IL8 were measured. Bacterial load was significantly higher in the mastitis samples and decreased after clinical symptoms disappeared. Bacterial diversity was lower in SAM milk sampl

SUBMITTER: Boix-Amoros A 

PROVIDER: S-EPMC7595153 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature