Enhancing natural killer cell function with gp41-targeting bispecific antibodies to combat HIV infection.

ABSTRACT:

Objective(s)

The aim of this study was to develop and evaluate the activity of bispecific antibodies (bsAbs) to enhance natural killer (NK) cell antibody-dependent cellular cytotoxicity (ADCC) against HIV-infected cells.

Design

These bsAbs are based on patient-derived antibodies targeting the conserved gp41 stump of HIV Env, and also incorporate a high-affinity single chain variable fragment (scFv) targeting the activating receptor CD16 on NK cells. Overall, we expect the bsAbs to provide increased affinity and avidity over their corresponding mAbs, allowing for improved ADCC activity against Env-expressing target cells.

Methods

bsAbs and their corresponding mAbs were expressed in 293T cells and purified. The binding of bsAbs and mAbs to their intended targets was det

SUBMITTER: Ramadoss NS 

PROVIDER: S-EPMC7595577 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature