MiR-10b inhibits migration and invasion of pancreatic ductal adenocarcinoma via regulating E2F7.

ABSTRACT:

Background

Abnormal microRNAs (miRNAs) expression is closely related to the development and poor prognosis of pancreatic ductal adenocarcinoma (PDAC). We aimed to elucidate the invasive mechanism and clinical significance of miR-10b in PDAC.

Methods

The RNA sequence data of pancreatic cancer were extracted from the TCGA database. R packages were performed to analyze the differential expression of RNAs. TargetScan, picTar, and miRanda were used to predict the target gene of miRNA. The expression level of the selected candidate was tested by western blot and RT-PCR in PDAC cells and tissues. Scrape and Transwell assays were determined the effect of candidate molecules on cell migration and invasion. The gain of function and loss of function was achieved by co-culture with mimi

SUBMITTER: Xu C 

PROVIDER: S-EPMC7595905 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature