Automated synthesis and preliminary evaluation of [¹⁸F]FDPA for cardiac inflammation imaging in rats after myocardial infarction.

ABSTRACT: A translocator protein 18 kDa targeted radiotracer, N,N-diethyl-2-(2-(4-[¹⁸F]fluorophenyl)-5,7-dimethylpyrazolo[1,5-a] pyrimidin-3-yl) acetamide ([¹⁸F]FDPA), was automated synthetized and evaluated for cardiac inflammation imaging. Various reaction conditions for an automated synthesis were systematically optimized. MicroPET/CT imaging were performed on normal rats and rats with myocardial infarction (MI). Normalized SUV ratios of [¹⁸F]FDPA to [¹³N]NH₃ (NSRs) in different regions were calculated to normalize the uptake of [¹⁸F]FDPA to perfusion. The amount of TBAOMs and the volume/proportion of water were crucial for synthesis. After optimization, the total synthesis time was 68 min. The non-decay corrected radiochemical yie

SUBMITTER: Mou T 

PROVIDER: S-EPMC7596090 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature