Expanding detection windows for discriminating single nucleotide variants using rationally designed DNA equalizer probes.

ABSTRACT: Combining experimental and simulation strategies to facilitate the design and operation of nucleic acid hybridization probes are highly important to both fundamental DNA nanotechnology and diverse biological/biomedical applications. Herein, we introduce a DNA equalizer gate (DEG) approach, a class of simulation-guided nucleic acid hybridization probes that drastically expand detection windows for discriminating single nucleotide variants in double-stranded DNA (dsDNA) via the user-definable transformation of the quantitative relationship between the detection signal and target concentrations. A thermodynamic-driven theoretical model was also developed, which quantitatively simulates and predicts the performance of DEG. The effectiveness of DEG for expanding detection windows and improving

SUBMITTER: Wang GA 

PROVIDER: S-EPMC7596233 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature