Ascorbate glutathione-dependent H2O2 scavenging is an important process in axillary bud outgrowth in rosebush.

ABSTRACT:

Background and aims

Branching is an important mechanism of plant shape establishment and the direct consequence of axillary bud outgrowth. Recently, hydrogen peroxide (H2O2) metabolism, known to be involved in plant growth and development, has been proposed to contribute to axillary bud outgrowth. However, the involvement of H2O2 in this process remains unclear.

Methods

We analysed the content of H2O2 during bud outgrowth and characterized its catabolism, both at the transcriptional level and in terms of its enzymatic activities, using RT-qPCR and spectrophotometric methods, respectively. In addition, we used in vitro culture to characterize the effects of H2O2 application and the reduced glutathione (GSH) synthesis inhibitor l-buthionine sulfoximine (BSO) on bud outgrowth i

SUBMITTER: Porcher A 

PROVIDER: S-EPMC7596375 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature