Enrichment of retinal ganglion and Muller glia progenitors from retinal organoids derived from human induced pluripotent stem cells - possibilities and current limitations.

ABSTRACT:

Background

Retinal organoids serve as excellent human-specific disease models for conditions affecting otherwise inaccessible retinal tissue from patients. They permit the isolation of key cell types affected in various eye diseases including retinal ganglion cells (RGCs) and Müller glia.

Aim

To refine human-induced pluripotent stem cells (hiPSCs) differentiated into three-dimensional (3D) retinal organoids to generate sufficient numbers of RGCs and Müller glia progenitors for downstream analyses.

Methods

In this study we described, evaluated, and refined methods with which to generate Müller glia and RGC progenitors, isolated them via magnetic-activated cell sorting, and assessed their lineage stability after prolonged 2D culture. Putative progenitor populatio

SUBMITTER: Freude KK 

PROVIDER: S-EPMC7596448 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature