Hypoxia-Inducible Factor α Subunits Regulate Tie2-Expressing Macrophages That Influence Tumor Oxygen and Perfusion in Murine Breast Cancer.

ABSTRACT: Tie2-expressing monocytes/macrophages (TEMs) are a distinct subset of proangiogenic monocytes selectively recruited to tumors in breast cancer. Because of the hypoxic nature of solid tumors, we investigated if oxygen, via hypoxia-inducible transcription factors HIF-1α and HIF-2α, regulates TEM function in the hypoxic tumor microenvironment. We orthotopically implanted PyMT breast tumor cells into the mammary fat pads of syngeneic LysMcre, HIF-1α ^fl/fl /LysMcre, or HIF-2α ^fl/fl /LysMcre mice and evaluated the tumor TEM population. There was no difference in the percentage of tumor macrophages among the mouse groups. In contrast, HIF-1α ^fl/fl /LysMcre mice had a significantly smaller percentage of tumor TEMs compared with control and HIF-

SUBMITTER: Steinberger KJ 

PROVIDER: S-EPMC7596922 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature