Effects of Low-Dose X-Ray on Cell Growth, Membrane Permeability, DNA Damage and Gene Transfer Efficiency.

ABSTRACT:

Background

We aimed to reveal if low dose X-rays would induce harmful or beneficial effect or dual response on biological cells and whether there are conditions the radiation can enhance gene transfer efficiency and promote cell growth but without damage to the cells.

Method

A systematic study was performed on the effects of Kilo-V and Mega-V X-rays on the cell morphology, viability, membrane permeability, DNA damage, and gene transfection of 293 T and CHO cells.

Results

The Kilo-V X-rays of very low doses from 0.01 to 0.04 Gray in principle didn't induce any significant change in cell morphology, growth, membrane permeability, and cause DNA damage. The Mega-V X-ray had a damage threshold between 1.0 and 1.5 Gray. The 0.25 Gray Mega-V-X-ray could promote cell growth a

SUBMITTER: Wang Z 

PROVIDER: S-EPMC7597563 | biostudies-literature | 2020 Oct-Dec

REPOSITORIES: biostudies-literature