Effects of different drying methods on smears of canine blood and effusion fluid.

ABSTRACT:

Background

Glass slide preparations from a variety of specimens (blood, masses, effusions) are commonly made as part of the diagnostic work-up, however the effects of various drying methods in veterinary practice and diagnostic laboratory settings is not clear.

Objective

Compare the effects of four drying methods on results of microscopic examination of canine blood smears and direct smears of pleural or peritoneal effusion fluid.

Methods

Twelve canine blood samples (6 from healthy dogs, 6 from sick dogs) and 6 canine peritoneal or pleural effusion samples. Four smears were prepared from each of the 18 samples and dried using the following methods: air-dry, hair dryer with or without heat, and heat block at 58 °C. Observers, blinded to the drying method, independently

SUBMITTER: De Witte FG 

PROVIDER: S-EPMC7597622 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature