Comparing sediment DNA extraction methods for assessing organic enrichment associated with marine aquaculture.

ABSTRACT: Marine sediments contain a high diversity of micro- and macro-organisms which are important in the functioning of biogeochemical cycles. Traditionally, anthropogenic perturbation has been investigated by identifying macro-organism responses along gradients. Environmental DNA (eDNA) analyses have recently been advocated as a rapid and cost-effective approach to measuring ecological impacts and efforts are underway to incorporate eDNA tools into monitoring. Before these methods can replace or complement existing methods, robustness and repeatability of each analytical step has to be demonstrated. One area that requires further investigation is the selection of sediment DNA extraction method. Environmental DNA sediment samples were obtained along a disturbance gradient adjacent to a Chinook (

SUBMITTER: Pearman JK 

PROVIDER: S-EPMC7597629 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature