Structure of the Visual Signaling Complex between Transducin and Phosphodiesterase 6.

ABSTRACT: Heterotrimeric G proteins communicate signals from activated G protein-coupled receptors to downstream effector proteins. In the phototransduction pathway responsible for vertebrate vision, the G protein-effector complex is composed of the GTP-bound transducin α subunit (Gα_T·GTP) and the cyclic GMP (cGMP) phosphodiesterase 6 (PDE6), which stimulates cGMP hydrolysis, leading to hyperpolarization of the photoreceptor cell. Here we report a cryo-electron microscopy (cryoEM) structure of PDE6 complexed to GTP-bound Gα_T. The structure reveals two Gα_T·GTP subunits engaging the PDE6 hetero-tetramer at both the PDE6 catalytic core and the PDEγ subunits, driving extensive rearrangements to relieve all inhibitory constraints on enzyme catalysis. Analysis of the confo

SUBMITTER: Gao Y 

PROVIDER: S-EPMC7597677 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature