Real-time microscopic assessment of fatty acid uptake kinetics in the human term placenta.

ABSTRACT:

Introduction

The placenta employs an efficient and selective fatty acid transport system to supply lipids for fetal development. Disruptions in placental fatty acid transport lead to restricted fetal growth along with cardiovascular and neurologic deficits. Nevertheless, little is known about the molecular mechanisms involved in human placental fatty acid trafficking during the initial steps of uptake, or the importance of fatty acid chain length in determining uptake rates.

Methods

We employed BODIPY fluorophore conjugated fatty acid analogues of three chain lengths, medium (BODIPY-C5), long (BODIPY-C12), and very-long (BODIPY-C16), to study fatty acid uptake in isolated human trophoblast and explants using confocal microscopy. The three BODIPY-labeled fatty acids were adde

SUBMITTER: Kolahi KS 

PROVIDER: S-EPMC7597898 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature