Multisubunit RNA Polymerases of Jumbo Bacteriophages.

ABSTRACT: Prokaryotic viruses with DNA genome longer than 200 kb are collectively referred to as "jumbo phages". Some representatives of this phylogenetically diverse group encode two DNA-dependent RNA polymerases (RNAPs)-a virion RNAP and a non-virion RNAP. In contrast to most other phage-encoded RNAPs, the jumbo phage RNAPs are multisubunit enzymes related to RNAPs of cellular organisms. Unlike all previously characterized multisubunit enzymes, jumbo phage RNAPs lack the universally conserved alpha subunits required for enzyme assembly. The mechanism of promoter recognition is also different from those used by cellular enzymes. For example, the AR9 phage non-virion RNAP requires uracils in its promoter and is able to initiate promoter-specific transcription from single-stranded DNA. Jumbo phages e

SUBMITTER: Sokolova ML 

PROVIDER: S-EPMC7598289 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature