Mannose-Decorated Dendritic Polyglycerol Nanocarriers Drive Antiparasitic Drugs To Leishmania infantum-Infected Macrophages.

ABSTRACT: Macrophages are hosts for intracellular pathogens involved in numerous diseases including leishmaniasis. They express surface receptors that may be exploited for specific drug-targeting. Recently, we developed a PEGylated dendritic polyglycerol-based conjugate (PG-PEG) that colocalizes with intracellular parasite. We hereby study the effect of surface decoration with mannose units on the conjugates' targeting ability toward leishmania intracellular parasites. Murine and human macrophages were exposed to fluorescently labeled mannosylated PG-PEG and uptake was quantified by flow cytometry analysis. Nanocarriers bearing five mannose units showed the highest uptake, which varied between 30 and 88% in the population in human and murine macrophages, respectively. The uptake was found to be depe

SUBMITTER: Vossen LI 

PROVIDER: S-EPMC7598597 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature