Myogenin is an essential regulator of adult myofibre growth and muscle stem cell homeostasis.

ABSTRACT: Growth and maintenance of skeletal muscle fibres depend on coordinated activation and return to quiescence of resident muscle stem cells (MuSCs). The transcription factor Myogenin (Myog) regulates myocyte fusion during development, but its role in adult myogenesis remains unclear. In contrast to mice, myog^-/-zebrafish are viable, but have hypotrophic muscles. By isolating adult myofibres with associated MuSCs, we found that myog^-/- myofibres have severely reduced nuclear number, but increased myonuclear domain size. Expression of fusogenic genes is decreased, Pax7 upregulated, MuSCs are fivefold more numerous and mis-positioned throughout the length of myog^-/-myofibres instead of localising at myofibre ends as in wild-type. Loss of Myog

SUBMITTER: Ganassi M 

PROVIDER: S-EPMC7599067 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature