YTHDF2 mediates the mRNA degradation of the tumor suppressors to induce AKT phosphorylation in N6-methyladenosine-dependent way in prostate cancer.

ABSTRACT:

Background

N6-methyladenosine (m⁶A) is the most abundant modification in mRNA of humans. Emerging evidence has supported the fact that m⁶A is comprehensively involved in various diseases especially cancers. As a crucial reader, YTHDF2 usually mediates the degradation of m⁶A-modified mRNAs in m⁶A-dependent way. However, the function and mechanisms of m⁶A especially YTHDF2 in prostate cancer (PCa) still remain elusive.

Methods

To investigate the functions and mechanisms of YTHDF2 in PCa, in vitro, in vivo biofunctional assays and epigenetics experiments were performed. Endogenous expression silencing of YTHDF2 and METTL3 was established with lentivirus-based shRNA technique. Colony formation, flow cytometry and trans-well

SUBMITTER: Li J 

PROVIDER: S-EPMC7599101 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature