Comparing the utility of in vivo transposon mutagenesis approaches in yeast species to infer gene essentiality.

ABSTRACT: In vivo transposon mutagenesis, coupled with deep sequencing, enables large-scale genome-wide mutant screens for genes essential in different growth conditions. We analyzed six large-scale studies performed on haploid strains of three yeast species (Saccharomyces cerevisiae, Schizosaccaromyces pombe, and Candida albicans), each mutagenized with two of three different heterologous transposons (AcDs, Hermes, and PiggyBac). Using a machine-learning approach, we evaluated the ability of the data to predict gene essentiality. Important data features included sufficient numbers and distribution of independent insertion events. All transposons showed some bias in insertion site preference because of jackpot events, and preferences for specific insertion sequences and short-distance vs long-distan

SUBMITTER: Levitan A 

PROVIDER: S-EPMC7599172 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature