Absolute quantum yield measurements of fluorescent proteins using a plasmonic nanocavity.

ABSTRACT: One of the key photophysical properties of fluorescent proteins that is most difficult to measure is the quantum yield. It describes how efficiently a fluorophore converts absorbed light into fluorescence. Its measurement using conventional methods become particularly problematic when it is unknown how many of the proposedly fluorescent molecules of a sample are indeed fluorescent (for example due to incomplete maturation, or the presence of photophysical dark states). Here, we use a plasmonic nanocavity-based method to measure absolute quantum yield values of commonly used fluorescent proteins. The method is calibration-free, does not require knowledge about maturation or potential dark states, and works on minute amounts of sample. The insensitivity of the nanocavity-based method to the

SUBMITTER: Ruhlandt D 

PROVIDER: S-EPMC7599333 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature