Identification and Analysis of microRNAs in Chlorella sorokiniana Using High-Throughput Sequencing.

ABSTRACT: Chlorella is a popular microalga with robust physiological and biochemical characteristics, which can be cultured under various conditions. The exploration of the small RNA content of Chlorella could improve strategies for the enhancement of metabolite production from this microalga. In this study, stress was introduced to the Chlorella sorokiniana culture to produce high-value metabolites such as carotenoids and phenolic content. The small RNA transcriptome of C. sorokiniana was sequenced, focusing on microRNA (miRNA) content. From the analysis, 98 miRNAs were identified in cultures subjected to normal and stress conditions. The functional analysis result showed that the miRNA targets found were most often involved in the biosynthesis of secondary metabolites, followed by protein metaboli

SUBMITTER: Azaman SNA 

PROVIDER: S-EPMC7599482 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature