Molecular Characterization of the Complete Coding Sequence of Olive Leaf Yellowing-Associated Virus.

ABSTRACT: Genome organization and phylogenetic relationships of olive leaf yellowing-associated virus (OLYaV) with other members of the Closteroviridae family were determined. The complete coding sequence of OLYaV was obtained by high throughput sequencing of total RNA from a 35-year-old olive tree (cv. Zarzaleña) from Brazil, showing olive leaf yellowing disease and deformations in the wood. This represents the first report of OLYaV in this country. A genomic sequence of 16,700 nt containing 11 open reading frames (ORFs) was recovered, representing the complete virus coding capacity. The knowledge of the nucleotide sequence of the genome including the gene that codes the coat protein will facilitate the development of diagnostic tests, which are limited so far to PCR-based methods targeting the HSP

SUBMITTER: Ruiz-Garcia AB 

PROVIDER: S-EPMC7599613 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature