A Novel Bioengineered Functional Motor Unit Platform to Study Neuromuscular Interaction.

ABSTRACT: In many neurodegenerative and muscular disorders, and loss of innervation in sarcopenia, improper reinnervation of muscle and dysfunction of the motor unit (MU) are key pathogenic features. In vivo studies of MUs are constrained due to difficulties isolating and extracting functional MUs, so there is a need for a simplified and reproducible system of engineered in vitro MUs. to develop and characterise a functional MU model in vitro, permitting the analysis of MU development and function. an immortalised human myoblast cell line was co-cultured with rat embryo spinal cord explants in a serum-free/growth fact media. MUs developed and the morphology of their components (neuromuscular junction (NMJ), myotubes and motor neurons) were characterised using immunocytochemistry, phase contrast and

SUBMITTER: Saini J 

PROVIDER: S-EPMC7599749 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature