Wolffia globosa-Mankai Plant-Based Protein Contains Bioactive Vitamin B₁₂ and Is Well Absorbed in Humans.

ABSTRACT:

Background

Rare plants that contain corrinoid compounds mostly comprise cobalamin analogues, which may compete with cobalamin (vitamin B₁₂ (B₁₂)) metabolism. We examined the presence of B₁₂ in a cultivated strain of an aquatic plant: Wolffia globosa (Mankai), and predicted functional pathways using gut-bioreactor, and the effects of long-term Mankai consumption as a partial meat substitute, on serum B₁₂ concentrations.

Methods

We used microbiological assay, liquid-chromatography/electrospray-ionization-tandem-mass-spectrometry (LC-MS/MS), and anoxic bioreactors for the B₁₂ experiments. We explored the effect of a green Mediterranean/low-meat diet, containing 100 g of frozen Mankai shake/day, on serum B₁₂

SUBMITTER: Sela I 

PROVIDER: S-EPMC7600829 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature