In Vitro Decoated Seed Germination and Seedling Development for Propagation of Wild Mandrake (Mandragora autumnalis Bertol.).

ABSTRACT: The establishment of an efficient in vitro propagation system for the conservation of the Mediterranean Mandragora autumnalis is highly desirable due to its scarcity, besides its potential medicinal and pharmacological properties. In a separate unpublished study, this species has proved to be resistant to laboratory plant regeneration from vegetative tissue cultures; therefore, an alternative decoated seed (i.e., endosperm enclosed the zygotic embryo) germination approach was conducted in this study. Pre-cold treatment of M. autumnalis seeds, removal of seed coats, and exogenous application of gibberellic acid (GA3) promoted in vitro seed germination and seedling emergence. In two separate experiments, approximately 10-27% of the germinated decoated seeds developed healthy seedlings within

SUBMITTER: Al-Ahmad H 

PROVIDER: S-EPMC7601314 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature