Salt-Tolerant Phenomena, Sequencing and Characterization of a GlyoxalaseI (Jojo-Gly I) Gene from Jojoba in Comparison with Other Glyoxalase I Genes.

ABSTRACT: Plant response to salt stress and the mechanism of salt tolerance have received major focus by plant biology researchers. Biotic stresses cause extensive losses in agricultural production globally, but abiotic stress causes significant increase in the methylglyoxal (MG) level of GlyoxalaseI (Gly I). Identification of salt-tolerant genes when characterizing their phenotypes will help to identify novel genes using polymerase chain reaction (PCR) to amplify the DNA coding region for glyoxalase I. This method is specific, requiring only genomic DNA and two pairs of PCR primers, and involving two successive PCR reactions. This method was used rapidly and easily identified glyoxalase I sequences as salt-tolerant genes from Jojoba (Simmondsia chinensis (Link) Schneider). In the present study, the

SUBMITTER: Mohasseb HAA 

PROVIDER: S-EPMC7601343 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature